Hello everyone, today I'll be sharing the procedure for using acid-fast mycobacterium fluorescent staining reagent. 

The kit includes solution A (Auramine O staining solution), destaining solution, and solution B. Instructions and Certificate

This product is mainly used for detecting mycobacteria, Nocardia, and other bacteria in paraffin sections of tissues, as well as samples such as sputum, bronchoalveolar lavage fluid, and pleural effusion using acid-fast fluorescent staining.

Rapid staining procedure for smears is as follows:

After preparing the sample smear, allow it to air dry (or slightly heat to fix the sample). Add 1 drop of solution A to the smear and stain at room temperature for 2-4 minutes.

After the timeout, rinse the specimen with water to remove solution A.

Add 1 drop of destaining solution and destain for 35-45 seconds. If the first destaining is incomplete, repeat the destaining process once.

After the timeout, rinse the specimen with water to remove the destaining solution.

Add 1 drop of solution B for counterstaining for 2-3 minutes. Do not overstain to avoid weakening the fluorescence.

After the timeout, rinse the specimen with water to remove solution B.

After rinsing, allow it to air dry naturally, or wipe away excess water from the slide with absorbent paper.

After drying, tilt the coverslip at a 45-degree angle and slowly cover it over the sample, then observe it under a microscope.

If you want to learn more about microscope observation, check out our previous videos.